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The use of metabolising enzymes in marine organisms as adaptive markers of environmental pollution (WAS AE0514 1/4) - AE1105

Description
R&D Summary Objectives:- Use molecular biological methods to examine specific enzymes in marine organisms which may be modified by the effects of environmental pollutants. Subsequently develop probes capable of assessing the biological effects of contaminants in marine species. Key customer purpose:- Enable new technological tools to be developed which will provide more cost effective monitoring and more robust sampling procedures for marine pollutants.
Objective
To apply the methodologies of molecular biology to investigate adaptive changes of xenobiotic metabolising enzymes in marine organisms as indicators of adverse biological effects resulting from environmental contamination. Probes which are specific for enzymes induced by polycyclic aromatic hydrocarbons (PAUs), planar polychlorobiphenyls (PCBs) and dioxins will be identified and used as tools to assess biological effects in marine species which have a wide spread distribution over Britain's continental shelf. The specific aims of the project are to: a)To use mammalian and piscine derived antibodies and cDNAs encoding various CYP isoforms, EHs and UDP GT to detect orthologs of these proteins in marine organisms and thus to identify species and proteins which are informative with respect to marine pollution. b)To prepare species specific cDNA and antibody probes for use with representative teleosts and crustaceans. c)To develop sensitive, simplified tests exploiting these probes suitable for routine screening of marine organisms.
Project Documents
• Final Report : AE1105: Final Report Use of metabolising enzymes as adaptive makers of environmental pollution in marine organisms   (2222k)
Time-Scale and Cost
From: 1993

To: 1997

Cost: £102,443
Contractor / Funded Organisations
University - Glasgow Caledonian
Keywords
              
Fields of Study
Environmental Protection - Marine